DNA Synthesis Reagents and Columns
New England Biolabs, Inc. Ph.D.™-12 Phage Display Peptide Library – 50 panning experiments
The Ph.D.-12 Phage Display Peptide Library contains a single tube of the Ph.D. -12 Phage Library. The Ph.D.-12 library is a combinatorial library of random 12-mer peptides fused to a minor coat protein (pIII) of M13 phage. The displayed peptide is expressed at the N-terminus of pIII. The library consists of approximately 1,000,000,000 electroporated (i.e., unique) sequences.
New England Biolabs, Inc. DNA Polymerase I, Large (Klenow) Fragment – 1000 units
DNA Polymerase I, Large (Klenow) Fragment is a proteolytic product of E. coli DNA Polymerase I which retains polymerization and 3' to 5' exonuclease activity, but has lost 5' to 3' exonuclease activity. Klenow retains the polymerization fidelity of the holoenzyme without degrading 5' termini.
New England Biolabs, Inc. Deep Vent DNA Polymerase – 1000 units
Deep Vent DNA Polymerase was the second high-fidelity thermophilic DNA polymerase available from New England Biolabs. The fidelity of Deep Vent DNA Polymerase is derived in part from an integral 3 5 proofreading exonuclease activity. Deep Vent is even more stable than Vent at temperatures of 95 to 100C.
New England Biolabs, Inc. RecJf – 5000 units
RecJf is a single-stranded DNA specific exonuclease that catalyzes the removal of deoxy-nucleotide monophosphates from DNA in the 5' to 3' direction. RecJf is a recombinant fusion protein of RecJ and maltose binding protein (MBP). It has the same enzymatic properties as wild-type RecJ. Fusion to MBP enhances RecJf solubility. When DNA containing a 22 base 5' extension is used as a substrate for RecJf, the resulting products are a mixture of DNA fragments that have blunt-ends, 5' extensions (1-5 nucleotides) and recessed 5' ends (1-8 nucleotides). RecJf does not require a 5' phosphate.
Quality Biological Inc Calcium Chloride 2 M, 100ML
Calcium Chloride 2 M is useful in the preparation of competent E.coli and transformation of bacteria.
Abcam 20(S)-Hydro x ycholesterol, Smoothened activator, 5MG
MW 402.65 g/mol. Allosteric activator of the Hedgehog (Hh) signaling pathway Smoothened (Smo) oncoprotein. Binds at a site distinct from the canonical cyclopamine binding site. Activates Hh signaling (EC₅₀ = ~ 3 μM for induction of Hh reporter gene transcription in NIH 3T3 cells). Also exhibits osteogenic activity and activates the liver X receptor (LXR).
The product is subject to the following: Abcam Restricted Use Statement